New
    Histamine Test Kits Histamine Test Kits Histamine Test Kits

Histamine Test Kits

food allergen testing

  • Fast and highly sensitive histamine analysis

Can we help you?

10002017

Model : 10002017

AgraQuant® Histamine

Quantitation Range : 0.5 - 50 ppb

Limit of Detection : 0.15 ppb

No. of Wells : 96


10002018

Model : 10002018

AgraQuant® Histamine Rapid

Quantitation Range : 3 - 300 ppm

Limit of Detection : 1 ppm

No. of Wells : 48




Test Kits for the Accurate Detection of Histamine

Histamine is an organic nitrogenous compound which naturally occurs in the human body, where it acts as neurotransmitter and is involved in immune responses and the regulation of physiological functions.

In an allergic reaction, the immune system releases a high level of histamine, leading to inflammatory reactions which are responsible for typical allergic symptoms. Those who are intolerant to histamine will suffer from similar reactions from an oral intake of histamine.

Histamine as indicator for bacterial growth

Histamine is produced by the decomposition of histidine caused by the growth of certain bacteria in fish and some other food products. Fish meal produced from materials which have been allowed to degrade prior to being processed may contain high levels of histamine and can thus be toxic.

Fish containing high levels of histamine has been associated with many examples of poisoning commonly referred to as “scombroid poisoning,” a major health problem for consumers. Quality control measures designed to minimize the occurrence of scombrotoxic fish require the determination of histamine levels in the range of approximately 10 to 200 ppm.

Fast and highly sensitive histamine analysis

The Romer Labs test kit portfolio covers 2 highly sensitive ELISA methods for the quantitative analysis of histamine in different food matrices.

AgraQuant® ELISA Tests

AgraQuant® Histamine test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. These test kits have been validated for the analysis of histamine in fresh fish, fish meal, cheese, sausage, milk, wine and champagne.

The AgraQuant® Histamine Rapid test kits are specifically developed for the detection of histamine in fresh, canned and salted fish, as well as in fish meal and fish in oil. The workflow of these test kit has been optimized for fish samples and allows for a much faster assay procedure.

Both AgraQuant® Histamine test kits are competitive ELISAs in a ready-to-use format to reduce handling errors and come with a set of 8 standards, a histamine coated plate, an acylation reagent, an acylation buffer, a wash buffer, a histamine antiserum conjugate, a substrate and a stop solution.

Results are measured with a specific ELISA reader, such as the StatFax® or the BioTek® reader.

Models:

AgraQuant® Histamine (10002017)

AgraQuant® Histamine Rapid (10002018)

AgraQuant® Histamine (10002017)

The AgraQuant® Histamine is an indirect competitive enzyme-linked immunosorbent assay (ELISA) designed for the quantification of histamine levels in foods, such as fish meal, fresh fish, milk, cheese, sausage and wine. This product is intended for laboratory use.

Properties

Item nameAGRAQUANT ALLERGEN - HISTAMINE, 96 WELLS
Storage temperature2°C - 8°C
Quantitation range0.5 - 50 ppb histamine
Limit of detection0.15 ppb histamine
Limit of quantitation0.5 ppb histamine
Number of wells96

Materials supplied

  • 96 non-coated reaction plate in a zip-lock bag
  • 96 histamine-coated microwells (12 eight-well strips) in a microwell holder sealed in a foil pouch
  • 6 ready-to-use standards (0; 0.5; 1.5; 5; 15 and 50 ppb)
  • 1 bottle of concentrated (5X) wash buffer
  • 1 bottle of conjugate solution
  • 1 bottle of substrate solution
  • 1 bottle of stop solution
  • 1 bottle of histamine antiserum
  • 2 bottles of acylation buffer
  • 2 bottles of acylation reagent
  • 1 bottle of control 1 (3 ppb ± 1 ppb)
  • 1 bottle of control 2 (10 ppb ± 3 ppb)
  • 1 piece of sealing tape for plate

Materials required but not supplied

  • Blender
  • analytical balance
  • 8-channel pipette
  • wash bottle
  • microwells/plate reader

AgraQuant® Histamine Rapid (10002018)

The AgraQuant® Histamine Rapid is an indirect competitive enzyme-linked immunosorbent assay (ELISA) designed for the quantification of histamine levels in foods, such as fish meal, canned fish, fresh fish, salted fish, and fish in oil. This product is intended for laboratory use.

Properties

Item nameAGRAQUANT ALLERGEN - HISTAMINE RAPID, 48 WELLS
Storage temperature2°C - 8°C
Quantitation range3 - 300 ppm histamine
Limit of detection1 ppm histamine
Limit of quantitation3 ppm histamine
Number of wells48

Materials supplied

  • 1 master block of 48 non-coated microwells in a zip-lock bag
  • 48 histamine-coated microwells (6 eight-well strips) in a microwell holder sealed in a foil pouch
  • 8 ready-to-use standards (0; 3; 10; 20; 30; 50; 100 and 300 ppm)
  • 1 bottle of concentrated (5X) wash buffer
  • 1 bottle of histamine antiserum conjugate
  • 1 bottle of substrate solution
  • 1 bottle of stop solution
  • 2 bottles of acylation buffer
  • 1 bottle of acylation reagent
  • 1 piece of sealing tape for plate

Materials required but not supplied

  • Blender
  • analytical balance
  • 8-channel pipette
  • wash bottle
  • microwells/plate reader