Casein Test Kit

Casein Test Kit

Casein refers to a family of proteins commonly found in mammalian milk. Approximately 80% of bovine milk proteins are caseins which are composed of α-, β- and κ-caseins. This protein fraction is the main component in cheese and is frequently used as a food additive.

Caseins also represent heat-stable allergens. Bovine milk is one of the most important allergenic food ingredients, especially for children. Consequently, the labeling of casein or milk is mandatory in many countries all over the world.

Although there are no legal threshold limits for casein, it is highly recommended that food manufacturers test for very low casein concentrations in order to protect allergic individuals and to avoid allergen-related recalls.

Fast and highly sensitive casein analysis

The Romer Labs casein test kit portfolio covers ELISAs and LFDs for the quantitative and qualitative analysis of casein in environmental samples, rinse waters and finished food products.

Detection of fining agents in wine

Casein is an organic compound frequently used as a fining agent in winemaking. In the fining process, casein is added to bind to suspended particles that will precipitate from the wine.

For wine samples, Romer Labs offers the validated AgraQuant® Casein ELISA and the AgraStrip® Casein LFD with a special wine extraction buffer specifically developed for wine analysis.

AgraStrip^® Lateral Flow Devices

The AgraStrip^® Casein test kits are ready-to-use lateral flow devices (LFD) for on-site testing. These immunological tests allow a rapid analysis of a wide range of food samples within only 11 minutes (including extraction).

AgraStrip^® LFDs represent a fast and simple qualitative strip test method in which specific antibodies labeled with colloidal gold are used for visual color development at a test and control zone.

The test kits come complete with strips, antibody coated incubation vials, extraction tubes, an extraction buffer, surface swabs and additional accessories.

Models:

AgraStrip^® Casein (10002040)

AgraStrip^® Wine Extraction Buffer (Casein) (10002039)

AgraQuant^® ELISA Tests

The AgraQuant^® Casein test kit is an accurate and reliable enzyme-linked immunosorbent assay (ELISA) in a quantitative format. Results are measured with a specific ELISA reader, such as the StatFax^® or the BioTek^® reader.

The AgraQuant^® Plus Casein test kit is specifically designed for a more efficient workflow. This test format uses unique extraction capsules that speed up the extraction of allergenic proteins, especially from processed matrices, to barely 1 minute.

Incubation times of the assay are reduced to 3 x 10 minutes, leading to a total assay time of 30 minutes for up to 38 samples.

Both AgraQuant^® Casein test kits are sandwich ELISAs in a ready-to-use format to reduce handling errors and come with a set of 5 standards, extraction solution /capsules, an antibody coated plate, a wash buffer, a conjugate, a substrate and a stop solution.

Models:

AgraQuant^® Plus Casein (10002042)

AgraQuant^® Casein (10002037)

AgraStrip^® Casein (10002040)

The AgraStrip^® Casein test kit is a lateral flow device (LFD) designed for the on-site detection of residues of the milk protein casein in raw materials, finished food products and beverages, rinse waters and environmental samples. They allow for the simple, fast and reliable qualitative detection of casein traces.

Properties

Materials supplied

• 1 tube with 10 AgraStrip^® Casein LFDs
• 1 foil pouch with 10 incubation vials containing antibodies
• 1 bottle of 35 mL ready-to-use extraction buffer with dropper cap
• 10 extraction tubes and caps
• 10 filtered dropper tips for the extraction tubes
• 10 sterile swabs with pre-scored tips
• 1 vial rack

Materials required but not supplied

• blender for solid samples

AgraQuant^® Plus Casein (10002042)

The AgraQuant^® Plus Casein is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative analysis of residues of the milk protein casein in food samples. It features a fast extraction procedure with a unique capsule technology. The total assay time is reduced to 30 minutes, with only 1 minute needed for the extraction procedure. The total assay time is reduced to 30 minutes, with only 1 minute needed for the extraction procedure. Samples can vary from raw to processed foods, from environmental swabs to rinse water. This product is intended for laboratory use.

Properties

Materials supplied

• 48 transfer wells sealed in a foil pouch
• 48 antibody-coated microwells sealed in a foil pouch
• 1 microwell strips frame
• 5 ready-to-use standards (0; 1; 5; 10 and 25 ppm)
• 1 bottle of 50 mL (20X) wash buffer
• 1 green-capped bottle of conjugate solution
• 1 blue-capped bottle of substrate solution
• 1 red-capped bottle of stop solution
• 1 bag of capsule extraction additive 1
• 1 bag of capsule extraction additive 2

Materials required but not supplied

• blender
• analytical balance
• 8-channel pipette
• wash bottle
• microwells/plate reader

AgraQuant^® Casein (10002037)

The AgraQuant^® Casein is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection of the milk protein casein in food samples. Samples can vary from raw to processed foods, from environmental swabs to rinse water. This product is intended for laboratory use.

Properties

Materials supplied

• 96 antibody-coated microwells in a microwell holder sealed in a foil pouch
• 5 concentrated (100X) standards (0; 0.2; 0.6; 2; and 6 ppm)
• 2 bottle of concentrated (5X) extraction buffer
• 1 bottle of concentrated (10X) wash buffer
• 1 green-capped bottle of conjugate solution
• 1 blue-capped bottle of substrate solution
• 1 red-capped bottle of stop solution

Materials required but not supplied

• blender
• analytical balance
• 8-channel pipette
• wash bottle
• microwells/plate reader